Metabolic compartmentation of pyruvate in the isolated perfused rat heart
نویسندگان
چکیده
منابع مشابه
Compartmentation of glycolysis and glycogenolysis in the perfused rat heart.
Developing methods that can detect compartmentation of metabolic pathways in intact tissues may be important for understanding energy demand and supply. In this study, we investigated compartmentation of glycolysis and glycogenolysis in the isolated perfused rat heart using (13)C NMR isotopomer analysis. Rat hearts previously depleted of myocardial glycogen were perfused with 5.5 mm [U-(13)C]gl...
متن کاملThe Regulation of Pyruvate Dehydrogenase in the Isolated Perfused Rat Heart*
The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...
متن کاملStudies on the regulation of pyruvate dehydrogenase in the isolated perfused rat heart.
The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...
متن کاملPyruvate carboxylation as an anaplerotic mechanism in the isolated perfused rat heart.
1. The role of pyruvate carboxylation in the net synthesis of tricarboxylic acid-cycle intermediates during acetate metabolism was studied in isolated rat hearts perfused with [1-14C]pyruvate. 2. The incorporation of the 14C label from [1-14C]pyruvate into the tricarboxylic acid-cycle intermediates points to a carbon input from pyruvate via enzymes in addition to pyruvate dehydrogenase and citr...
متن کاملMetabolic Imaging of the Perfused Rat Heart Using Hyperpolarized [1-13C]Pyruvate
Introduction : The recent development of liquid state Dynamic Nuclear Polarization (DNP) techniques has dramatically increased the signal available from C MRS experiments and has opened up new possibilities for metabolic imaging of the heart [1]. Oxidative decarboxylation of DNP hyperpolarized [1-C]pyruvate, mediated by the pyruvate dehydrogenase (PDH) complex, is a critical reaction that produ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0264-6021
DOI: 10.1042/bj2100193